mouse il 1β levels  (Proteintech)

Journal: Cell reports

Article Title: Non-canonical autophosphorylation of RIPK1 drives timely pyroptosis to control Yersinia infection

doi: 10.1016/j.celrep.2024.114641

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Article Snippet: Cell free supernatants were analyzed for IL-1β (DY401) protein levels using the DuoSet ELISA kit from R&D Systems.

Techniques: Virus, Recombinant, Staining, Electroporation, CRISPR, Enzyme-linked Immunosorbent Assay, In Situ, Expressing, Sequencing, Mutagenesis, Software

Journal: Foods

Article Title: Benzyl Isothiocyanate and Resveratrol Synergistically Alleviate Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.3390/foods13132078

Figure Lengend Snippet: Synergistic antioxidant activities of BITC and RES. DPPH ( A ) and ABTS• + ( B ) radical scavenging capacities. BITC, benzyl isothiocyanate. RES, resveratrol. Different superscript letters in the graph (a, b, c, and d) indicate significant differences ( p < 0.05).

Article Snippet: Mouse IL-6, IL-1β, and TNF-α level and IL-10 enzyme-associated immunosorbent test (ELISA) kits (Neobioscience Technology, Shenzhen, China) were used to quantify the serum IL-6, IL-1β, TNF-α, and IL-10 concentrations via ELISA.

Techniques:

Journal: Foods

Article Title: Benzyl Isothiocyanate and Resveratrol Synergistically Alleviate Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.3390/foods13132078

Figure Lengend Snippet: Synergistic effects of BITC and RES on iNOS and MPO levels in colonic tissues and cytokine levels in serum. ( A ) iNOS, ( B ) MPO, ( C ) TNF-α, ( D ) IL-1β, ( E ) IL-6, and ( F ) IL-10. The data are expressed as the mean ± SD ( n = 6). Bars with different superscripts letters are significantly different.

Article Snippet: Mouse IL-6, IL-1β, and TNF-α level and IL-10 enzyme-associated immunosorbent test (ELISA) kits (Neobioscience Technology, Shenzhen, China) were used to quantify the serum IL-6, IL-1β, TNF-α, and IL-10 concentrations via ELISA.

Techniques:

Journal: Preventive Nutrition and Food Science

Article Title: Effects of Haskap ( Lonicera caerulea L.) Extracts against Oxidative Stress and Inflammation in RAW 264.7 Cells

doi: 10.3746/pnf.2024.29.2.146

Figure Lengend Snippet: Effects of 200 μg/mL of Lonicera caerulea L. ethanol extract (LCEE) (or L. caerulea L. water extract, LCWE) on the viability of RAW 264.7 cells. Mean values in the same bar graph (a) are not significantly different ( P >0.05) according to Tukey’s test.

Article Snippet: Determination of IL-6, IL-1β, and TNF-α levels in LPS-induced RAW 264.7 cells treated with LCEE and LCWE : After the medium was removed from the RAW 264.7 cell inflammatory injury model, 200 μL of LCEE and LCWE (200 μg/mL) was added to each well, along with 2 mL of fresh DMEM to continue incubation for 24 h. Enzyme-linked immunosorbent assay was used to determine IL-6 (catalog number: M6000B), TNF-α (catalog number: MTA00B), and IL-1β levels (catalog number: MLB00C) in accordance with the manufacturer’s recommendations (Bio-Techne Corp.).

Techniques:

Journal: Preventive Nutrition and Food Science

Article Title: Effects of Haskap ( Lonicera caerulea L.) Extracts against Oxidative Stress and Inflammation in RAW 264.7 Cells

doi: 10.3746/pnf.2024.29.2.146

Figure Lengend Snippet: Effects of Lonicera caerulea L. ethanol extract (LCEE) and L. caerulea L. water extract (LCWE) on the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α levels of hydrogen peroxide (H 2 O 2 )-induced RAW 264.7 cells. Mean values with different letters in the same bar graph (a-d) are significantly different ( P <0.05) according to Tukey’s test. Vc, vitamin C.

Article Snippet: Determination of IL-6, IL-1β, and TNF-α levels in LPS-induced RAW 264.7 cells treated with LCEE and LCWE : After the medium was removed from the RAW 264.7 cell inflammatory injury model, 200 μL of LCEE and LCWE (200 μg/mL) was added to each well, along with 2 mL of fresh DMEM to continue incubation for 24 h. Enzyme-linked immunosorbent assay was used to determine IL-6 (catalog number: M6000B), TNF-α (catalog number: MTA00B), and IL-1β levels (catalog number: MLB00C) in accordance with the manufacturer’s recommendations (Bio-Techne Corp.).

Techniques: